BOAR VESICULAR SECRETION PROTEINS: FURTHER COMPARISONS WITH SEMINAL PLASMA PROTEINS

Fertility of boar spermatozoa after freezing in the absence of seminal vesicular proteins.

A comparison of spermatozoa from intact boars and from boars without seminal vesicles, frozen in various diluents, was made to determine any differences in sperm quality after thawing and the conception rates of gilts. Sperm samples were frozen at -196 degrees C. 50 semen pellets were thawed after 1-3 weeks storage and assessed for postthaw motility at 37 degrees C. The percentage of eosinop...

Protein analysis of boar seminal plasma proteins with protective effect during low-temperature storage of spermatozoa

The present study aimed to investigate the effect of different seminal plasma proteins (SPPs) on boar spermatozoa functional characteristics. We investigated the putative protective effect of SSPs on sperm cells motility and velocity, as well as on the integrity of the plasma membrane (PM) during low-temperature storage at 4 °C. SPPs fractions were obtained and purified by gel permeation chroma...

Association of oxidative status and semen characteristics with seminal plasma proteins of buffalo semen

To study the influence of season on oxidative status of buffalo semen and their association with semen characteristics and seminal plasma proteins, ejaculates were collected twice a week in winter, summer and rainy seasons from six adult Bhadawari buffalo bulls. The neat semen was analyzed for semen characteristics immediately after collection and oxidative status viz. lipid peroxidation (LPO),...

Proteomics of boar seminal plasma

Extensive dataset of boar seminal plasma proteome displaying putative reproductive functions of identified proteins

A complete proteomic profile of seminal plasma (SP) remains challenging, particularly in porcine. The data reports on the analysis of boar SP-proteins by using a combination of SEC, 1-D SDS PAGE and NanoLC-ESI-MS/MS from 33 pooled SP-samples (11 boars, 3 ejaculates/boar). A complete dataset of the 536 SP-proteins identified and validated with confidence ≥95% (Unused Score >1.3) and a false disc...